9. There have been other genome editing systems that were able to modify
DNA in specific organisms such as mice or flies. Why is the CRISPR/Cas9
system an improvement compared to these older technologies?
Type your answer here.
Critical thinking
10. To test whether Cas9 will cut where you predicted, you will
run your resulting DNA fragments on an agarose gel.
Insert a sketch below of what you expect to see when the
following samples are run on a gel.
● If needed, a template can be accessed here.
● Label each lane with the sample name.
⎻ Lane 1: Ladder
⎻ Lane 2: Uncut DNA
⎻ Lane 3: Cas9+gRNA1
⎻ Lane 4: Cas9+gRNA2.
● Use the image of the Fast DNA Ladder 3 to the right
as a reference to label the approximate size of the
DNA fragments (in bp) in your gel.
Chopped! Using CRISPR/Cas9 to cut DNA Lab. Student E-worksheet™.
Release: September 2023. © 2023 by miniPCR bio
TM
