Pre-lab study questions
Review
1. What is genome editing?
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2. Name two reasons why CRISPR/Cas9 is considered such a powerful
genome editing tool.
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3. What are the two main parts that make up the CRISPR/Cas9 system?
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4. Cas9 is a nuclease. What does being a nuclease tell you about the
function of Cas9?
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5. How does a Cas9 nuclease differ from most other nucleases?
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6. A guide RNA (gRNA) has two regions: the scaffold and the spacer.
a. What is the function of the scaffold region in a gRNA?
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b. What is the function of the spacer region in a gRNA?
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7. Explain why the CRISPR/Cas9 system isspecific.
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8. Explain how the CRISPR/Cas9 system is programmable.
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Chopped! Using CRISPR/Cas9 to cut DNA Lab. Student E-worksheet™.
Release: September 2023. © 2023 by miniPCR bio
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