11. In today's experiment, you will set up four different reactions, each
containing a different combination of reagents. This will allow you to
investigate the function of each component in the CRISPR/Cas9 system.
The table below outlines what you will add to each tube.
Tube A Tube B Tube C Tube D
Cas9 - ✓ ✓ ✓
Guide RNA - - gRNA1 gRNA2
DNA Sample ✓ ✓ ✓ ✓
Use the information from the table above to predict your experimental results.
Tube A Tube B Tube C Tube D
Will the DNA
be cut in this
reaction?
Type your
answer here.
Type your
answer here.
Type your
answer here.
Type your
answer here.
Justification
Type your
answer here.
Type your
answer here.
Type your
answer here.
Type your
answer here.
Predicted
DNA
fragment
size(s)
*from
questions 2
and 3 in the
pre-lab
activity
Type your
answer here.
Type your
answer here.
Type your
answer here.
Type your
answer here.
Chopped! Using CRISPR/Cas9 to cut DNA Lab. Student E-worksheet™.
Release: September 2023. © 2023 by miniPCR bio
TM
