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miniPCR bio TM Chopped! Using CRISPR/Cas9 to cut DNA - Instructor's and Student's Guide Version: 1.0 - Release: May 2022 - © 2022 by miniPCR bio™ P./52 Instructor's Guide Students who demonstrate understanding can: HS-LS3-2 Make and defend a claim based on evidence that inheritable genetic variations may result from (1) new genetic combinations through meiosis, (2) viable errors occurring during replication, and/or (3) mutations caused by environmental factors. Science and Engineering Practice Disciplinary Core Ideas Crosscutting Concepts • Asking Questions and Defining Problems • Developing and Using Models • Planning and Carrying Out Investigations • Analyzing and Interpreting Data Using Mathematics and Computational Thinking • Constructing Explanations and Designing Solutions • Engaging in Argument from Evidence • Obtaining, Evaluating, and Communicating Information LS1.A: From Molecules to Organisms: Structures and Processes LS3.B: Variation of Traits • Patterns • Cause and Effect • Scale, Proportion And Quantity Structure and Function • Stability and Change • Interdependence of Science, Engineering, and Technology • Influence of Engineerings, Technology, and Science on Society and the Natural World Standards alignment Next Generation Science Standards Common Core ELA/Literacy Standards RST.9-10.1 Cite specific textual evidence to support analysis of science and technical texts, attending to the precise details of explanations or descriptions. RST.9-10.3 Follow precisely a complex multistep procedure when carrying out experiments, taking measurements, or performing technical tasks, attending to special cases or exceptions defined in the text. RST.9-10.4 Determine the meaning of symbols, key terms, and other domain-specific words and phrases as they are used in a specific scientific or technical context relevant to grades 9-10 texts and topics. RST.9-10.5 Analyze the structure of the relationships among concepts in a text, including relationships among key terms (e.g., force, friction, reaction force, energy). RST.9-10.9 Compare and contrast findings presented in a text to those from other sources (including their own experiments), noting when the findings support or contradict previous explanations or accounts. WHST.9-10.1 Write arguments focused on discipline-specific content. WHST.9-10.2 Write informative/explanatory texts, including the narration of historical events, scientific procedures/ experiments, or technical processes. WHST.9-10.9 Draw evidence from informational texts to support analysis, reflection, and research. For simplicity, this activity has been aligned to high school NGSS and grades 9-10 Common Core standards.