miniPCR bio
TM
Chopped! Using CRISPR/Cas9 to cut DNA - Instructor's and Student's Guide
Version: 1.0 - Release: May 2022 - © 2022 by miniPCR bio™
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Instructor's Guide
Learning goals and skills developed
Student Learning Goals - students will:
• Determine how gRNAs and Cas9 work together to target and cut specific DNA sequences
• Test how different guide RNAs effect the function of the CRISPR/Cas9 system
• Analyze DNA sequences to predict where a given guide RNA will target and use their
experimental results to evaluate their predictions
Scientific Inquiry Skills - students will:
• Identify or pose a testable question
• Formulate hypotheses
• Identify dependent and independent variables and appropriate experimental controls
• Follow detailed experimental protocols
• Create tables or graphs to present their results
• Interpret data presented in a chart or table
• Use data to evaluate a hypothesis
• Make a claim based in scientific evidence
• Use reasoning to justify a scientific claim
Molecular Biology Skills:
• Micropipetting
• In vitro CRISPR/Cas9 reactions
• Preparation of agarose gels
• Agarose gel DNA electrophoresis
• Staining, visualization, and molecular weight analysis of DNA fragments
