miniPCR bio
TM
Chopped! Using CRISPR/Cas9 to cut DNA - Instructor's and Student's Guide
Version: 1.0 - Release: May 2022 - © 2022 by miniPCR bio™
P./53
Instructor's Guide
Ordering information
To order Chopped! kits, you can:
Call (781)-990-8PCR
email us at orders@minipcr.com
visit https://www.minipcr.com
Materials are sufficient for 8 lab groups
All components should be kept frozen at -20 °C for long-term storage
Reagents must be used within 12 months of shipment
Other reagents needed
• Agarose (electrophoresis grade)
• DNA stain (e.g., SeeGreen
TM
or GelGreen
®
)
• Gel electrophoresis buffer (e.g., 1X TBE)
• Distilled or deionized H
2
O (to prepare 1X TBE buffer)
Note: Agarose, DNA stain, and TBE buffer are available at minipcr.com as part of the Learning Lab
Companion Kit (KT-1510-01)
Chopped! Using CRISPR/Cas9 to cut DNA (catalog no. KT-1801-01) contains the following
reagents:
• Nuclease-Free Water
• Reaction Buffer
• 5X Cas9 Nuclease Concentrate
• gRNA1
• gRNA2
• DNA Sample
• Proteinase K solution
• Fast DNA Ladder 3
