miniPCR bio
TM
Chopped! Using CRISPR/Cas9 to cut DNA - Instructor's and Student's Guide
Version: 1.0 - Release: May 2022 - © 2022 by miniPCR bio™
P./50
Instructor's Guide
Additional student supports
At miniPCR bio™, we are committed to preparing students to be successful in the laboratory through
high quality curriculum and training. We have created an extensive set of resources to help your
students succeed in molecular biology techniques, all of which are available for free download at the
miniPCR bio™ tutorials page of our website.
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https://www.minipcr.com/tutorials/
Those activities most relevant to this lab are listed below.
Micropipetting: Video and activity resources to train students in the basic use of a micropipette.
Gel electrophoresis: Video and worksheet activity instructing students on the fundamentals and
practice of agarose gel electrophoresis.
In addition, the following supports are provided for this lab:
DNAdots on CRISPR/Cas9: This lab gives an introduction to how CRISPR/Cas9 is able to target
specific DNA sequences. To learn how this can be applied to genome editing, students can read
more about CRISPR/Cas9 and its applications. DNAdots include articles and study questions. Find
this article at: https://dnadots.minipcr.com/dnadots/crisprcas-9.
CRISPR/Cas9 paper model - examining snail coiling: Use a paper model to dive into a fun example
of basic science research. Students model research where scientists used CRISPR/Cas9 to determine
the genetic control of the direction that a snail's shell spirals (Abe and Kuroda, 2019).
https://links.minipcr.com/crispr_snail
CRISPR/Cas9 paper model - treating sickle cell disease: Use a paper-based activity to model
cutting-edge therapy. The extension contains all the background information on sickle cell disease
and hemoglobin for students to understand the mechanism underlying this proposed treatment for
sickle cell disease.
Note: This activity is more conceptually advanced than the snail paper model.
https://links.minipcr.com/crispr_sicklecell
