miniPCR bio
TM
Chopped! Using CRISPR/Cas9 to cut DNA - Student's Guide
Version: 1.0 - Release: May 2022 - © 2022 by miniPCR bio™
Student's Guide
P./35
4. Fill out the table below to compare your predictions from the pre-lab activity to your
experimental observations.
• Under the "Predicted DNA fragment size(s)" column, copy down your predicted
fragment size(s) from the table in the pre-lab questions (page 29).
• Under "Observed DNA fragment size(s)", write down the observed fragment size(s) from
the reaction (refer to your gel image or sketch from question 1 on page 34).
Critical thinking
1. Tube A and Tube B are included in this experiment as controls. Explain the role that each
control plays in this experiment.
a. Tube A:
b. Tube B:
2. Assume you set up a tube that contained a gRNA and the DNA sample, but no Cas9
nuclease. Would you expect the DNA sample to be cut? Justify your response.
gRNA
used
Predicted DNA fragment size(s) Observed DNA fragment size(s)
Tube A
None
Tube B
None
Tube C
gRNA1
Tube D
gRNA2
