miniPCR bio
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Chopped! Using CRISPR/Cas9 to cut DNA - Student's Guide
Version: 1.0 - Release: May 2022 - © 2022 by miniPCR bio™
Student's Guide
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3. Compare the results from the four different reactions. Which tube(s) best demonstrate(s)
that Cas9 will only target and cut DNA in the presence of a guide RNA? Justify your answer.
4. Compare the results from the four different reactions. Which tube(s) best demonstrate(s)
the function of the gRNAs to guide the Cas9 nuclease to target a specific DNA sequence?
Justify your answer.
5. This lab demonstrates that the same Cas9 nuclease can be used to cut a DNA sample at two
different locations, just by changing the gRNA used. In your own words, describe how this
makes the CRISPR/Cas9 system useful as a genome editing tool.
6. In the lab portion of this activity, there were billions of DNA molecules in each tube. Each
DNA strand was an identical 3,142 base pairs long and contained the correct target sequence
for the gRNA. Compare this to the DNA in a living cell. How might you expect targeting in a
cell to be different from what you did in this experiment?
