miniPCR bio™ Electrophoresis Forensics Lab. Wrongfully Convicted? Instructor's and Student's Guide
Version: 1.1 - Release February 2022 - © 2022 by miniPCR bio™
Student's Guide
P./17
Gel electrophoresis - Running the gel
These instructions are designed for use with blueGel™ electrophoresis system by miniPCR bio™. If
using another electrophoresis system, these instructions may need to be adjusted according to the
manufacturer's instructions.
1. Place the gel tray containing your gel in the buffer
chamber
• Ensure that the clear buffer chamber is inside
the blueGel™ electrophoresis system.
• The wells of the gel should be on the same
side as the negative electrode, away from the
power button.
2. Add 30 ml of 1X TBE electrophoresis buffer
• The buffer should just cover the gel and fill
the wells.
• Ensure that there are no air bubbles in the
wells (shake the gel gently if bubbles need to
be dislodged).
3. Load samples onto the gel in the following
sequence
• Lane 1: 10 μl Fast DNA Ladder 1
• Lane 2: 10 μl victim DNA
• Lane 3: 10 μl J.M. DNA
• Lane 4: 10 μl DNA Evidence 1
• Lane 5: 10 μl DNA Evidence 2
Note: Samples already contain loading dye.
4. Place the orange cover on the blueGel™ electrophoresis system
• To prevent fogging, make sure that ClearView™ spray has
been evenly applied to the inside of the orange cover.
• Match the positive and negative electrode signs on the
orange lid with the corresponding positive and negative signs
on the blue base.
• The orange lid should sit flush with the blue base using little
force.
Protective gloves and eyewear should be worn for the entirety of this experiment.
Base
Buffer
chamber
Gel tray
