miniPCR bio™ Electrophoresis Forensics Lab. Wrongfully Convicted? Instructor's and Student's Guide
Version: 1.1 - Release February 2022 - © 2022 by miniPCR bio™
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Instructor's Guide
C. Prepare for gel electrophoresis
• Prepare 1X TBE buffer.
- TBE buffer is often provided as liquid concentrate or powder.
- Follow manufacturer's instructions to prepare 1X TBE buffer solution.
- Volume to prepare depends on the method used to prepare gels; see "Important
Note" below.
• Gels can be poured in advance of the class.
- This lab requires running and visualizing DNA samples on a 2% agarose gel with a
fluorescent DNA stain (e.g., SeeGreen
TM
or GelGreen
®
).
- Pre-poured gels can be stored at ambient temperature, in a sealed container or
wrapped in plastic wrap, and protected from light for up to three days.
• Have the banding pattern of the Fast DNA Ladder 1 handy (page 18) to help interpret the
electrophoresis results.
IMPORTANT NOTE: There are several ways to prepare agarose gels.
• Scan the QR code for detailed instructions on how to prepare
agarose gels.
• Both written and video instructions are available.
www.minipcr.com/agarose-gel/
