miniPCR bio™ Electrophoresis Forensics Lab. Wrongfully Convicted? Instructor's and Student's Guide
Version: 1.1 - Release February 2022 - © 2022 by miniPCR bio™
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Instructor's Guide
Lab setup
A. Dispense reagents and supplies
• Thaw tubes containing the DNA samples by placing
them on a rack or benchtop at room temperature
• For each lab group, dispense the following reagents
into five labeled 1.7 ml microtubes
- Victim DNA 12 μl
- J.M. DNA 12 μl
- DNA Evidence 1 12 μl
- DNA Evidence 2 12 μl
- Fast DNA Ladder 1 12 μl
The following activities can be carried out by the instructor ahead of class. Reagents are sufficient
to be used with 8 student groups. Reagents are stable at room temperature for several hours during
setup and the lab experiment, but should otherwise remain frozen for long-term storage.
Check At the start of this experiment, every lab group should have: Amount
DNA samples for analysis
• Victim DNA
• J.M. DNA
• DNA Evidence 1
• DNA Evidence 2
12 μl of each sample
DNA ladder 12 μl
2-20 μl micropipette 1
Micropipette tips 5
Five wells in an electrophoresis gel
DNA
evidence
2
DNA
evidence
1
J.M.
DNA
Victim
DNA
Defrost tubes
B. Distribute supplies and reagents to lab groups
Gloves and protective eyewear should be worn for the entirety of this lab.
up to 8
number
of groups
V
12 μl
Victim DNA
J.M.
12 μl
J.M. DNA
E
12 μl
DNA
evidence 1
E2
12 μl
DNA
evidence 2
L
12 μl
Fast DNA
Ladder 1
