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MiniPCR Wrongfully Convicted Activity - Instructor Guide

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miniPCR bio™ Electrophoresis Forensics Lab. Wrongfully Convicted? Instructor's and Student's Guide Version: 1.1 - Release February 2022 - © 2022 by miniPCR bio™ P./5 Instructor's Guide Materials needed (cont.) Reagents and supplies Amount needed per lab group Teacher's checklist Horizontal gel electrophoresis apparatus: e.g. blueGel™ electrophoresis system 1 If sharing gels, reserve 1 lane for ladder and 4 lanes for each group Blue light transilluminator *Note: A blue light transilluminator is integrated in the blueGel electrophoresis system. Micropipettes • 2-20 μl: one per lab group 1 Disposable micropipette tips At least 5 per group Microcentrifuge (optional; only needed to collect liquid at tube bottom) Distilled water for making agarose gels and diluting TBE buffer 50 ml per gel Flask or beaker to dissolve agarose Microwave or hot plate to dissolve agarose Other supplies: • Disposable laboratory gloves • Protective eyewear • Permanent marker • Cup to dispose of tips Available at miniPCR.com Supplied by teacher

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