miniPCR bio
TM
Dye Electrophoresis Lab: Molecular Rainbow - Student's Guide
Version: 1.0 - Release: April 2022 - © 2022 by miniPCR bio™
Student's Guide
P./17
Laboratory guide
Pouring gels (before or during class period)
• Gels can be prepared up to five days ahead of time and stored at room
temperature if placed in an airtight container.
• These instructions are designed for use with the pre-weighed Agarose
Tabs
TM
provided in the lab kit.
• One Agarose Tab
TM
will yield one gel for use in either a Bandit
TM
or
blueGel™ electrophoresis system by miniPCR bio™.
• If using a different electrophoresis system, these instructions may need
to be adjusted according to the manufacturer's instructions. You can use
gels of any percentage between 1-2%.
Protective gloves and eyewear should be worn for the entirety of this experiment.
A. Prepare 1X TBE buffer (to be completed by teacher in advance)
• Prepare at least 60 ml of buffer for every Bandit
TM
or blueGel™ electrophoresis system you plan
to use.
• 30 ml of the buffer will be used to make your gel and 30 ml will be used as running buffer.
• Refer to page 6 for detailed instructions on preparing 1X TBE buffer.
B. Prepare an agarose solution
• Obtain a heat-resistant container such as a glass
Erlenmeyer flask or beaker that is at least three
times the volume you wish to add.
• Combine 30 ml room temperature 1X TBE buffer and
one Agarose Tab
TM
for each gel you plan to pour.
• Allow the tabs to soak until they fully disintegrate
(this could take a few minutes).
• Swirl the flask or beaker to ensure the tabs have
fully disintegrated before heating.
30 ml
1X TBE
buer
50
40
30
20
10
Agarose Tab
TM
If using a Bandit
TM
STEM Electrophoresis Kit, detailed
instructions for pouring and running gels can be found
on page 29
