miniPCR bio
TM
Dye Electrophoresis Lab: Molecular Rainbow - Instructor's and Student's Guide
Version: 1.0 - Release: April 2022 - © 2022 by miniPCR bio™
Student's Guide
P./18
D. Set up your gel casting system with the comb in the center position
• You will need six lanes per gel.
• If using a Bandit
TM
STEM electrophoresis kit, make sure Electrodams™ are firmly in place before
pouring the gel. Refer to page 29 for detailed assembly instructions.
• If using a different electrophoresis system, refer to the manufacturer's instructions for how to
set up your particular gel casting system.
E. Pour the agarose solution into the prepared casting
platform with a gel tray and comb
• The agarose solution should cover the bottom of
the gel tray and the bottom 3 mm of the comb
(roughly the bottom 1/3 of the comb).
• Note: Because this lab uses colored dyes as
experimental samples, there is no need to add
DNA stain.
F. Allow gel to solidify completely
• Gel is ready when cool and firm to the touch.
• Gels will typically be ready in about 10 minutes.
• Gels can be stored in an airtight container at
room temperature for up to five days before use.
• You can remove the comb and disassemble the
gel casting apparatus before storing the gel.
Prepare an agarose gel with
comb in center position
C. Heat solution
• Expect to heat for about 60 seconds per 30 ml of liquid in
a standard microwave.
• Heat until the solution boils and continue until agarose is
fully dissolved. No agarose particles should remain.
30 ml
1X TBE
buer
50
40
30
20
10
Agarose Tab
TM
Caution: The solution may boil over the top of some containers. The solution will be very hot.
