miniPCR bio
TM
Dye Electrophoresis Lab: Molecular Rainbow - Instructor's and Student's Guide
Version: 1.0 - Release: April 2022 - © 2022 by miniPCR bio™
Student's Guide
P./19
Cover gel with 1X
TBE buffer
Running the gel
1. Submerge your gel in enough 1X TBE buffer
to just cover the gel and fill the wells
• If using a Bandit
TM
STEM electrophoresis kit,
your gel is already in the buffer chamber.
Ensure that the Electrodams
TM
are in the
correct orientation and have the electrode
wires threaded (refer to page 33 for detailed
instructions).
• If using a Bandit
TM
or blueGel
TM
electrophoresis
system you will need approximately 30 ml of 1X
TBE buffer.
2. Use a micropipette to load samples onto the gel in
the following sequence
• Lane 1: 10 μl Red Dye
• Lane 2: 10 μl Orange Dye
• Lane 3: 10 μl Yellow Dye
• Lane 4: 10 μl Green Dye
• Lane 5: 10 μl Blue Dye
• Lane 6: 10 μl Purple Dye
3. Connect the electrodes and turn on your gel
electrophoresis system
• If using a Bandit
TM
STEM electrophoresis
kit, refer to page 33 for detailed assembly
instructions.
4. Conduct electrophoresis for 15-25 minutes
• Longer electrophoresis times will result in better separation.
• Placing the gel over a white background will make it easier
to see your results.
