2. Fill out the table below to document your experimental observations.
Under the "Observation" column, write down whether or not you
observed that any of the DNA was cut and explain whether this makes
sense based on what was in the reaction.
Observation: Was any of the
DNA cut in this reaction?
(yes or no)
Explanation: Does this make
sense based on what was in the
reaction? Explain your reasoning
Tube A
Type your answer here. Type your answer here.
Tube B
Type your answer here. Type your answer here.
Tube C
Type your answer here. Type your answer here.
Tube D
Type your answer here. Type your answer here.
3. Fill out the table below to compare your predictions from the pre-lab
activity to your experimental observations.
● Under the "Predicted DNA fragment size(s)" column, copy down
your predicted fragment size(s) from the table in the pre-lab
questions.
● Under the "Observed DNA fragment size(s)" column, write down
the observed fragment size(s) from your experiment.
gRNA
used
Predicted DNA fragment
size(s)
Observed DNA fragment
size(s)
Tube A None
Type your answer here. Type your answer here.
Tube B None
Type your answer here. Type your answer here.
Tube C gRNA1
Type your answer here. Type your answer here.
Tube D gRNA2
Type your answer here. Type your answer here.
Chopped! Using CRISPR/Cas9 to cut DNA Lab. Student E-worksheet™.
Release: September 2023. © 2023 by miniPCR bio
TM
