17. Based on your answer to the previous questions, explain whether you
think a restriction enzyme with a six-base-pair recognition sequence
would make a good genome editing tool.
Type your answer here.
18. Now, calculate about how many times you would expect the Cas9 enzyme
to find and cut a specific 20-base-pair sequence in the 3.2 billion
(3,200,000,000) base pair-long human genome. You should use a
calculator, but show your work.
Type your answer here.
19. Your answer to the previous question should be a number that is less than
1. This tells us that it is not likely for that specific 20-base sequence to
appear even once in the 3.2 billion (3,200,000,000) base-pair sequence.
This means that any 20-base sequence within the human genome will
likely only appear once, if at all. Explain why this makes the CRISPR/Cas9
system a good genome editing tool.
Type your answer here.
Chopped! Using CRISPR/Cas9 to cut DNA Lab. Student E-worksheet™.
Release: September 2023. © 2023 by miniPCR bio
TM
