Pre-labactivity
Predicting where the CRISPR/Cas9 system will cut
● Youwillperformthispre-labactivitywithahands-onpapermodelofthetwo
guide RNAs and the Target DNA sequence.
● Instructions for using the paper models and determining where the guide
RNAs cut can be found in the lab guide on page 18.
1. Note the position of the circled base pair for each of the gRNAs below.
● gRNA 1: Type your answer here.
● gRNA 2: Type your answer here.
2. The entire length of the DNA is 3,142 bp. Based on where you predict the
Cas9 nuclease to cut the DNA, predict the length of the two DNA
fragments that will be produced by gRNA 1.
● Fragment 1: Type your answer here.
● Fragment 2: Type your answer here.
3. The entire length of the DNA is 3,142 bp. Based on where you predict the
Cas9 nuclease to cut the DNA, predict the length of the two DNA
fragments that will be produced by gRNA 2.
● Fragment 1: Type your answer here.
● Fragment 2: Type your answer here.
4. In this activity, you used a cutout of a guide RNA to find the cut site in a
printed DNA sequence. Which part of the CRISPR/Cas system did YOU
most closely represent in this activity? Explain your reasoning.
Type your answer here.
Chopped! Using CRISPR/Cas9 to cut DNA Lab. Student E-worksheet™.
Release: September 2023. © 2023 by miniPCR bio
TM
