miniPCR bio
TM
Chopped! Using CRISPR/Cas9 to cut DNA - Instructor's and Student's Guide
Version: 1.0 - Release: May 2022 - © 2022 by miniPCR bio™
P./6
Instructor's Guide
Materials needed (cont.)
Supplied by teacher
Reagents
and supplies Amount needed
Teacher's
checklist
37 °C heat source
• e.g., miniPCR
®
thermal cycler, incubator, or
water bath
1
Four reactions per group
Machines can be shared between
groups
Horizontal gel electrophoresis apparatus
e.g. blueGel™ electrophoresis system
1
If sharing gels, reserve one lane
for DNA ladder and 4 lanes for
each group
Blue light transilluminator
*Note: A blue light transilluminator is integrated
in the blueGel™ electrophoresis system
1
Can be shared
between groups
Micropipettes
• 2-20 μl: one per lab group
• 20-200 μl: one for the teacher to dispense
reagents
1
Disposable micropipette tips At least 24 per group
(19 for reaction setup and 5 for
electrophoresis)
Distilled water
for making agarose gels and diluting TBE buffer
at least 760 ml
Heat resistant flask or beaker
to dissolve agarose
Microwave or hot plate
to dissolve agarose
Other supplies:
• Disposable laboratory gloves
• Protective eyewear
• Permanent marker
• Cup to dispose of tips
• Crushed ice
Available
at
miniPCR.com
