miniPCR bio
TM
Chopped! Using CRISPR/Cas9 to cut DNA - Instructor's and Student's Guide
Version: 1.0 - Release: May 2022 - © 2022 by miniPCR bio™
P./5
Instructor's Guide
Reagents
and supplies
Amount
provided
in kit
Amount
needed per
lab group Storage
Teacher's
checklist
All-in-one Agarose Tabs
TM
8 One tab makes
two agarose
gels (one 1% gel
is needed per
group)
Room
temp.,
protected
from light
TBE electrophoresis buffer
• 1X working solution
Supplied
as liquid
concentrate
or powder
Sufficient for
600 ml of
1X working
solution
30 ml 1X
solution per
blueGel™
system
Room
temp.
PCR tubes (0.2 ml) 100 8-12 (for
dispensing
reagents and
performing
reactions—see
note)
Plastic microtubes (1.7 ml) 50
0-4 (for
performing
reactions—see
note)
Sold separately in Learning Lab Companion Kit
(KT-1510-01)
Note: This lab requires reagents for running and visualizing DNA samples on a 1% agarose gel with
DNA stain. One Learning Lab Companion Kit provides enough electrophoresis reagents for at least
eight groups when using the blueGel
TM
electrophoresis system.
Note: Reactions must be incubated at 37 °C for 15 minutes. Students may use either 0.2 ml or 1.7 ml plastic tubes for their
reactions, but should use the size that is compatible with your 37 °C heat source.
