miniPCR bio
TM
Chopped! Using CRISPR/Cas9 to cut DNA - Instructor's and Student's Guide
Version: 1.0 - Release: May 2022 - © 2022 by miniPCR bio™
P./4
Instructor's Guide
Reagents
and supplies
Amount
provided
in kit
Amount
needed per
lab group Storage*
Teacher's
checklist
Nuclease-Free Water 1 ml 20 μl
Freezer
Reaction Buffer 225 μl 25 μl
Freezer
5X Cas9 Nuclease
Concentrate
40 μl
concentrated
enzyme
20 μl
diluted
enzyme**
Freezer
gRNA1
(Guide RNA 1)
90 μl 10 μl
Freezer
gRNA2
(Guide RNA 2)
90 μl 10 μl
Freezer
DNA Sample 225 μl 25 μl
Freezer
Proteinase K Solution 180 μl 20 μl
Freezer
Fast DNA Ladder 3 150 μl 15 μl
Freezer
Materials needed
Supplied in kit (KT-1801-01)
*IMPORTANT NOTE: The Chopped! reagents will be shipped overnight on ice and will need to be stored in
the freezer immediately upon arrival. We strongly recommend storing the reagents in the coldest part of
the freezer where temperature fluctuations are minimal (e.g., in the back of the freezer instead of the freezer
door).
**The Cas9 Nuclease has greater stability when stored at higher concentrations. For this reason, you will
need to dilute the enzyme shortly before use. See "Preparation for in vitro CRISPR/Cas reaction" on page 7
for more details.
Keep the Cas9 Nuclease on ice at all times.
