miniPCR bio
TM
Chopped! Using CRISPR/Cas9 to cut DNA - Student's Guide
Version: 1.0 - Release: May 2022 - © 2022 by miniPCR bio™
Student's Guide
P./26
5. Press the "Run" button
• Check that the green light beside the power button remains illuminated.
6. Conduct electrophoresis for 20-30 minutes
• The colored dye should progress to about half the length of the gel.
• Longer electrophoresis times will result in better size resolution.
4. Place the orange cover on the blueGel™ electrophoresis
system
• To prevent fogging, make sure that ClearView™ spray has
been evenly applied to the inside of the orange cover.
• Match the positive and negative electrode signs on the
orange lid with the corresponding positive and negative
signs on the blue base.
• The electrodes of the lid should be aligned with the metal
leads on the base.
• The orange lid should sit flush with the blue base using
little force.
Gel electrophoresis – Visualizing results
1. Press the "light bulb" button to turn on the blueGel™
transilluminator
• For best viewing, dim lights or use Fold-a-View™ photo
documentation hood with a smartphone camera.
• Gels may be viewed at the end of the run or periodically
throughout the run.
• If the image appears hazy, wipe off the inside of the
orange cover and reapply ClearView™ spray.
2. Ensure that there is sufficient DNA band resolution
• Run the gel longer if needed to increase resolution.
3. Document your results
• Place Fold-a-View™ photo documentation hood on the
blueGel™ electrophoresis system to take a picture with a
smartphone or other digital camera.
• Compare the bands from the DNA samples to the ladder
to obtain size estimates.
Fast DNA Ladder 3.
