miniPCR bio
TM
Chopped! Using CRISPR/Cas9 to cut DNA - Student's Guide
Version: 1.0 - Release: May 2022 - © 2022 by miniPCR bio™
Student's Guide
P./25
4. Pour the agarose solution into the prepared casting
platform with a gel tray and comb
• The agarose solution should cover the bottom of
the gel tray and the bottom 3 mm of the comb
(roughly the bottom 1/3 of the comb).
5. Allow gel to solidify completely and remove the
comb by pulling firmly upwards
• Gels will typically be ready in about 10 minutes.
• Gel is ready when cool and firm to the touch.
Gel electrophoresis: Running the
gel
These instructions are designed for use with blueGel™
electrophoresis system by miniPCR bio™. If using another
electrophoresis system, these instructions may need to be
adjusted according to the manufacturer's instructions.
1. Place the gel tray containing your gel in the buffer
chamber
• Ensure that the clear buffer chamber is inside
the blueGel™ electrophoresis system.
• The wells of the gel should be on the same side
as the negative electrode, away from the power
button.
2. Add 30 ml of 1X TBE electrophoresis buffer
• The buffer should just cover the gel and fill the
wells.
• Ensure that there are no air bubbles in the wells
(shake the gel gently if bubbles need to be
dislodged).
3. Load samples onto the gel in the following sequence
• Lane 1: 10 μl Fast DNA Ladder 3
• Lane 2: 15 μl of Tube A
• Lane 3: 15 μl of Tube B
• Lane 4: 15 μl of Tube C
• Lane 5: 15 μl of Tube D
Base
Buffer
chamber
Gel tray
