miniPCR bio
TM
Chopped! Using CRISPR/Cas9 to cut DNA - Student's Guide
Version: 1.0 - Release: May 2022 - © 2022 by miniPCR bio™
Student's Guide
P./24
This lab uses 1% agarose gels. You will need four lanes per group, plus
one lane for the ladder. If groups are sharing gels, a single lane for
ladder is sufficient.
Gels can be prepared up to three days ahead of time and stored at
ambient temperature, covered in air-tight plastic wrap and protected
from light.
These instructions are designed for use with the blueGel™ electrophoresis system by miniPCR bio™.
If using another electrophoresis system, these instructions may need to be adjusted according to the
manufacturer's instructions.
1. Prepare 1X TBE buffer (to be completed by teacher in advance)
• TBE buffer is often provided as liquid concentrate or powder.
• Follow manufacturer's instructions to prepare 1X TBE buffer solution.
2. Prepare a clean and dry casting platform
with a gel tray and comb
• Place the clear gel tray in the white
casting platform.
• Place a well-forming comb at the top
of the gel tray.
3. Prepare a 1% agarose solution using the
method indicated by your instructor
Gel tray
Casting
platform
Comb
Gel electrophoresis: Pouring gels (before or during
class period)
IMPORTANT NOTE: There are several ways to prepare agarose gels
• Scan the QR code for detailed instructions on how to prepare
agarose gels.
• Both written and video instructions are available.
• The video demonstrates making a 2% gel as an example. Use the
volumes specified in the written instructions for making a 1% gel to
prepare gels for this lab.
www.minipcr.com/agarose-gel/
