Typical Biotech/Cloning Workflow
Cloning describes the processes used to create an exact genetic replica of another cell, tissue, or organism.
Researchers use a Biotech/cloning Workflow like this one that documents the sequence of activities in the
cloning processes.
DNA Extraction
Isolates the DNA from the host
culture to generate a purified
DNA sample.
Tissue Harvesting
PCR (or qPCR)
Polymerase Chain Reaction
amplifies your gene of interest
in your DNA sample.
Restriction Digestion
Restriction digestion cuts your amplified
DNA's ends at points specific to certain
enzymes. Preps for ligation into plasmid
or further targets gene of interest.
Ligation
Ligation glues the newly chopped
pieces of DNA with compatible ends
into one plasmid.
Gel Electrophoresis
Runs your DNA fragments on a matrix that separates them
based on size (the larger the fragment, the slower it moves).
It can be used as a QC step or to visualize the result.
Transformation
Transformation incorporates the
DNA into the host's genome, typically
bacterial so that the host can make
millions of copies as it grows.
Cell Culture
Sequencing
(NGS, Sanger)
Determining the order and frequency
of the four nucleotide bases (adenine,
guanine, cytosine, and thymine) in a
sequence.
ELISA
"Enzyme-Linked-ImmunoSorbent-As-
say; used to determine the quantity of
a protein, antibody, antigen, or other
material in a sample.
Proteomics
The large scale study of proteins and
the genetics behind their function.
Blotting
The transfer and immobilization of
proteins onto a surface. Commonly
used for the detection and identifica-
tion of various proteins, antigens, or
other macromolecules.
