Ward's World Infographics and Guides

Biotech_Cloning Workflow

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DNA Extraction • Microcentrifuge • Extraction kits • Micro Tube Racks • Micropipettes Tissue Harvesting PCR (or qPCR) • Polymerase • .2mL PCR tubes • Thermal cycler • Tube coolers Restriction Digestion • Restriction enzymes • Thermal cycler • Micropipettes Ligation • Water bath • Tube coolers • Micropipettes Gel Electrophoresis Transformation • Ice bucket • Water bath • Bacterial host • Shaking incubators Cell Culture Sequencing (NGS, Sanger) Determining the order and frequency of the four nucleotide bases (adenine, guanine, cytosine, and thymine) in a sequence. ELISA "Enzyme-Linked-ImmunoSorbent-As- say; used to determine the quantity of a protein, antibody, antigen, or other material in a sample. Proteomics The large scale study of proteins and the genetics behind their function. Blotting The transfer and immobilization of proteins onto a surface. Commonly used for the detection and identifica- tion of various proteins, antigens, or other macromolecules. Typical Biotech/Cloning Workflow Use this diagram to help plan which products you'll need to support your biotech lessons for the year. Recommended Products • Power supply • Gel box • TAE or TBE buffer • Agarose tablets or powder + ward ' s science Visit our Biotechnology resources page for additional ideas for stocking your lab.

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