miniPCR bio™ Electrophoresis Forensics Lab. Wrongfully Convicted? Instructor's and Student's Guide
Version: 1.1 - Release February 2022 - © 2022 by miniPCR bio™
Student's Guide
P./15
P. 10
Laboratory guide
Gel electrophoresis - Pouring gels (before or during
class period)
Gels can be prepared up to three days ahead of time and stored at
ambient temperature, covered in air-tight plastic wrap and protected
from light.
You will need 4 lanes plus one lane for ladder per group. If groups are
sharing gels, a single lane for ladder is sufficient.
These instructions are designed for use with the blueGel™ electrophoresis system by miniPCR
bio™. If using another electrophoresis system, these instructions may need to be adjusted according
to the manufacturer's instructions.
1. Prepare 1X TBE buffer (to be completed by teacher in advance)
• TBE buffer is often provided as liquid concentrate or powder.
• Follow manufacturer's instructions to prepare 1X TBE buffer solution.
2. Prepare a clean and dry casting platform with a gel tray
and comb
• Place the clear gel tray in the white casting platform.
• Place a well-forming comb at the top of the gel tray.
3. Prepare a 2% agarose solution with a fluorescent DNA
stain (e.g., SeeGreen
TM
or GelGreen
®
) using the method
indicated by your instructor
Protective gloves and eyewear should be worn for the entirety of this experiment.
Gel tray
Casting
platform
Comb
IMPORTANT NOTE: There are several ways to prepare agarose gels.
• Scan the QR code for detailed instructions on how to prepare
agarose gels.
• Both written and video instructions are available.
www.minipcr.com/agarose-gel/
