miniPCR bio™ Electrophoresis Forensics Lab. Wrongfully Convicted? Instructor's and Student's Guide
Version: 1.1 - Release February 2022 - © 2022 by miniPCR bio™
Student's Guide
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5. Press the "Run" button
• Check that the green light beside the power button remains illuminated.
6. Conduct electrophoresis for 15-25 minutes
• Note: Check the progress of your samples every 10 minutes to monitor the migration of
your DNA samples.
• Longer electrophoresis times will result in better separation of similar sized DNA
fragments. However, if run too long, small DNA fragments can run off the end of the gel
or lose fluorescence.
Gel electrophoresis – Visualizing
results
1. Press the "light bulb" button to turn on the blueGel™
transilluminator
• For best viewing, dim lights or use Fold-a-View™
photo documentation hood with a smartphone
camera.
• Gels may be viewed at the end of the run or
periodically throughout the run.
• If image appears hazy, wipe off the inside of the
orange cover and reapply ClearView™ spray.
2. Ensure that there is sufficient DNA band resolution in the
200-400 bp range of the Fast DNA Ladder 1
• Run the gel longer if needed to increase resolution.
3. Document your results
• Compare the bands from the DNA samples to the
ladder to obtain size estimates.
• Place Fold-a-View™ photo documentation hood on
the blueGel™ electrophoresis system to take a picture
with a smartphone or other digital camera.
bp Fast DNA
Ladder 1
1200
700
500
300
200
100
