miniPCR bio
TM
Dye Electrophoresis Lab: Molecular Rainbow - Instructor's and Student's Guide
Version: 1.0 - Release: April 2022 - © 2022 by miniPCR bio™
P./34
Instructor's Guide
Plug alligator
cable into right side
of Bandit
TM
circuit controller
Plug USB-C cable
into left side of
Bandit
TM
circuit
controller
Plug into USB-C power
source of choice
D. Run the gel
1. Add 30 ml of 1X TBE electrophoresis buffer
• The buffer should just cover the gel and fill the
wells, in addition to the spaces between the
Electrodams
TM
and the gel.
• Ensure that there are no air bubbles in the
wells (shake the gel gently if bubbles need to be
dislodged).
2. Use a micropipette to load samples onto the gel in the
order specified in the lab guide
• Lane 1: 10 μl Red Dye
• Lane 2: 10 μl Orange Dye
• Lane 3: 10 μl Yellow Dye
• Lane 4: 10 μl Green Dye
• Lane 5: 10 μl Blue Dye
• Lane 6: 10 μl Purple Dye
3. Connect the power cords
• Plug the wire connected to the alligator clips into the round port on the right side of the
Bandit
TM
circuit controller.
• Plug the USB-C cord into the USB-C port on the left side of the Bandit
TM
circuit controller.
• Plug the other end of the USB-C cord into your power source of choice.
• Note: USB-C power source is provided by the user.
• A small light on the right side of the Bandit
TM
circuit controller will illuminate to indicate
that the power is on.
• Look for bubbles on the electrode wires to verify that all the wires are connected and
current is flowing.
4. Conduct electrophoresis for 15-25 minutes or until adequate separation is achieved
