miniPCR bio
TM
Dye Electrophoresis Lab: Molecular Rainbow - Instructor's and Student's Guide
Version: 1.0 - Release: April 2022 - © 2022 by miniPCR bio™
P./32
Instructor's Guide
1. Remove the comb, comb supports, and Electrodams
TM
by pulling firmly upwards
2. Prepare the electrodes
• Note: The electrode wire can remain attached to the Electrodams
TM
for several uses. If
the electrode wires are already in place, proceed to step 3.
• If the Electrodams
TM
do not already have wire attached, cut two approximately 15 cm (6
inch) pieces of electrode wire from the included spool.
• Repeat for the other Electrodam
TM
, but thread the
electrode wire from the opposite side so that the
long free ends of the electrode wires extend in
opposite directions.
Remove comb Remove comb supports Remove Electrodams
TM
C. Set up Bandit
TM
for gel run
- Starting from the tall
side of the Electrodam
TM
with a channel present,
thread the wire through
one of the small holes.
- You only need about
2 cm of wire to come
through on the other
side.
- Flip the Electrodam
TM
over.
- Twist the short free end
of the wire that you just
threaded through the
hole around the longer
piece of the wire to
prevent it from slipping
back through the hole.
- Flip the Electrodam
TM
back
over so you are looking
at the side with the
channel again.
- Thread the loose end of
the wire through the
small hole on the other
side of the Electrodam
TM
.
- Slowly pull the long free
end of the wire until is
it taut and the electrode
wire sits flush in the
channel.
