Advanced questions
5. When performing gel electrophoresis, you can determine which bands
have more DNA based on their relative brightness; when comparing two
bands of the same size, the brighter band has more DNA.
You may wish to consult with other lab groups in order to evaluate more data.
a. Compare your results from Tube C versus Tube D. Which one had
the brighter band of uncut DNA?
Type your answer here.
b. What does this suggest about the efficiency of the two gRNAs used
in this experiment? Explain your answer.
Type your answer here.
6. Scientists often have to test multiple gRNAs to find the most efficient one
for their experiment. Comparing the two gRNAs you have tested, if you
had to advise a scientist which gRNA was better for cutting this sample of
DNA, which one would you choose? Justify your answer.
Type your answer here.
Chopped! Using CRISPR/Cas9 to cut DNA Lab. Student E-worksheet™.
Release: September 2023. © 2023 by miniPCR bio
TM
