miniPCR bio
TM
Chopped! Using CRISPR/Cas9 to cut DNA - Student's Guide
Version: 1.0 - Release: May 2022 - © 2022 by miniPCR bio™
Student's Guide
P./20
The diagram below represents the entire DNA fragment. Using your calculations, clearly mark where
you think Cas9 will cut this DNA when paired with gRNA1.
In this activity you used a cutout of a guide RNA to find the cut site in a printed DNA sequence.
Which part of the CRISPR/Cas9 system did YOU most closely represent in this activity?
Explain your reasoning.
gRNA2: Cas9 cuts at base pair (from 5, above) ______
The entire length of the DNA is 3,142 bp. Based on where you predict the Cas9 nuclease to cut the
DNA, predict the length of the two DNA fragments that will be produced.
Fragment 1: ___________ bp (same number as question 5 above)
Fragment 2: __________ bp (subtract Fragment 1 from 3,142 bp)
The diagram below represents the entire DNA fragment. Using your calculations, clearly mark where
you think Cas9 will cut this DNA when paired with gRNA2.
DNA sample
Base
pairs
Section you analyzed
0 200 400 600 800 1000 1200 1400 1600 1800 2000 2200 2400 2600 2800 3000
DNA sample
Base
pairs
Section you analyzed
0 200 400 600 800 1000 1200 1400 1600 1800 2000 2200 2400 2600 2800 3000
