miniPCR bio
TM
Chopped! Using CRISPR/Cas9 to cut DNA - Instructor's and Student's Guide
Version: 1.0 - Release: May 2022 - © 2022 by miniPCR bio™
P./10
Instructor's Guide
IMPORTANT NOTE: There are several ways to prepare agarose gels
• Scan the QR code for detailed instructions on how to prepare
agarose gels.
• Both written and video instructions are available.
• The video demonstrates making a 2% gel as an example. Use the
volumes specified in the written instructions for making a 1% gel to
prepare gels for this lab.
Preparation for gel electrophoresis
• Prepare 1X TBE buffer.
- TBE buffer is often provided as liquid concentrate or powder.
- Follow manufacturer's instructions to prepare 1X TBE buffer solution.
- Volume to prepare depends on the method used to prepare gels; see "Important Note"
below.
• Gels can be poured in advance of the class, up to three days ahead. You will need 1% agarose
gels that contain a fluorescent DNA stain.
- Pre-poured gels can be stored at ambient temperature, in a sealed container or wrapped
in plastic wrap, and protected from light for up to three days.
• Have the banding pattern of the Fast DNA Ladder 3 handy (page 26) to help interpret the
electrophoresis results.
• Distribute supplies and reagents to lab groups.
Check At the start of this experiment, every lab group should have: Amount
Reactions from previous section of lab 4 reactions
Fast DNA Ladder 3
15 μl
2-20 μl micropipette 1
Micropipette tips At least 5
5 wells in an electrophoresis gel
www.minipcr.com/agarose-gel/
