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MiniPCR Chopped! CRISPR Activity - Instructor Guide

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miniPCR bio TM Chopped! Using CRISPR/Cas9 to cut DNA - Student's Guide Version: 1.0 - Release: May 2022 - © 2022 by miniPCR bio™ Student's Guide P./38 CER Table Fill in the table based on your results from the lab. Use the rubric on the next page to guide your answers. Question: Which component of the Cas9/gRNA complex is responsible for directing where the target DNA will be cut? Claim Make a clear statement that answers the above question. Evidence Provide data from the lab that supports your claim (hint: you may want to consult other lab groups' results, in order to have more data to evaluate) Reasoning Explain clearly why the data you presented supports your claim. Include the underlying scientific principles that link your evidence to your claim.

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