miniPCR bio
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Chopped! Using CRISPR/Cas9 to cut DNA - Student's Guide
Version: 1.0 - Release: May 2022 - © 2022 by miniPCR bio™
Student's Guide
P./27
Pre-lab study questions
Review
1. What is genome editing?
2. Name two reasons why CRISPR/Cas9 is considered such a powerful genome editing tool.
3. What are the two main parts that make up the CRISPR/Cas9 system?
4. Cas9 is a nuclease. What does being a nuclease tell you about the function of Cas9?
5. How does a Cas9 nuclease differ from most other nucleases?
6. A guide RNA (gRNA) has two regions: the scaffold and the spacer.
A. What is the function of the scaffold region in a gRNA?
B. What is the function of the spacer region in a gRNA?
