miniPCR bio
TM
Chopped! Using CRISPR/Cas9 to cut DNA - Instructor's and Student's Guide
Version: 1.0 - Release: May 2022 - © 2022 by miniPCR bio™
P./3
Instructor's Guide
At a glance
Planning your time
-
Take a close-up look at the molecular machinery that makes CRISPR/Cas such a powerful genome
editing tool.
Students will use the CRISPR/Cas9 system in vitro to cut DNA in a test tube. By using different guide
RNAs, students will investigate how the Cas9 nuclease can be programmed to target specific DNA
sequences.
TECHNIQUES
Micropipetting
Gel electrophoresis
Gene targeting
TOPICS
Biotechnology
CRISPR/Cas9
LEVEL
General high school
Advanced high school
College
WHAT YOU NEED
Micropipettes
Gel electrophoresis
and visualization
system
AP CONNECTION
AP Biology units
1.6, 3.1, 6.1, 6.8
Skills and Practices
1.A-1.C, 2.A-2.D,
3.A-3.E, 5-A, 6.A-6.E
Help your students build proficiency in pipetting and understanding the biological
concepts involved with additional instructional videos, worksheets, and activities
available at: minipcr.com/tutorials/
Taking it further
• Visit minipcr.com/crispr to access the complete miniPCR bio™ CRISPR/Cas
resource library
Additional
supports
-
* Allow an additional 25 minutes if
students will be preparing gels in class
For answers to the lab study questions, email answers@minipcr.com. Please include the name of the lab, as well as your
name, school, and title in the body of the email.
Analyze gRNA and
DNA sequences
Mix and incubate
reactions
Analyze results with
gel electrophoresis
SINGLE CLASS PERIOD: 90 MINUTES
OPTIONAL PRE-LAB: 30 MINUTES FIRST CLASS PERIOD: 45 MINUTES SECOND CLASS PERIOD: 45 MINUTES*
OR
