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Agar Art — An Intro to Microbiology
Submitted by: Lauren H.
Recommended Grade Level(s):
Appropriate for: Grade 9 Biology
Time Requirements:
Activity Time: 3 x 47 min class periods
Teaching Topics & Concepts:
• What cells are and how to distinguish between eukaryotes and prokaryotes.
• How bacteria grow and reproduce.
• Relate to Real Life: MRSA and Nosocomial infections
National Science Standards Alignment
Tennessee State Standards: BIO1.LS1: From Molecules to Organisms: Structures and Processes.
BIO2.LS4: Biological Change: Unity and Diversity.
Pre-Instruction:
• Teacher: Pre-made TSA agar plates, gloves, goggles, apron, proper PPE, bacteria samples, heat lamp, sterile plastic inoculating
loops, instruction manual, worksheets for students.
• Technology: SmartBoard to show instructions on how to create a proper streak plate.
• Student: writing utensil, hair tie (long hair), goggles, gloves, proper PPE equipment.
Materials:
For class: Agar plate
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• Microorganisms (microbes):
1. Bacillus subtilis (cream to brown)
2. Micrococcus luteus (yellow)
3. Chromobacterium violaceum (violet)
4. Escherichia coli (colorless)
5. Micrococcus roseus (pink)
6. Proteus mirabilis
7. Pseudomonas aeruginosa strains having variuous
pigments i.e. pyocyanin, pyoverdin, prorubrin
and pyomelanin
8. Serratia marcescens (pink or orange)
9. Staphylococcus aureus (yellow)
10. Vibrio fischeri (bioluminescent)
11. Yeast species — which are fungi — used include
Saccharomyces cerevisiae (yellow-white)
12. Aspergillus flavus (yellow-green spores)
13. Aspergillus ochraceus (yellow)
14. Aureobasidium pullulans (black)
15. Candida species (whitish)
16. Candida albicans (whitish buff )
17. Cladosporium herbarum (brown to black)
18. Cladosporium resinae
19. Epicoccum nigrum (yellow, orange, red, brown & black)
20. Fusarium species
21. Rhodotorula species and Scopulariopsis brevicaulis
22. Protist species used include Euglena gracilis
(photosynthetic, green) and
23. Physarum polycephalum (yellow-green)
• Inoculating loop
• Bunsen burner
• Incubator
