Ward's World Activity Guides

+ ward ' s science Page 2 Objectives: • Create a model for the major events of the eukaryotic cell cycle, including mitosis. Compare and contrast the rates of cell division in various eukaryotic cell types in multicellular organisms. • Utilize a model of a cell plasma membrane to compare the various types of cellular transport and test predictions about the movement of molecules into or out of a cell based on the homeostasis of energy and matter in cells. • Create a model of photosynthesis demonstrating the net flow of matter and energy into a cell. Use the model to explain energy transfer from light energy into stored chemical energy in the product. • Create a model of aerobic respiration demonstrating flow of matter and energy out of a cell. Use the model to explain energy transfer mechanisms. Compare aerobic respiration to alternative processes of glucose metabolism. • Use models of viruses, prokaryotes, and eukaryotes to ask questions about characteristics of living things and analyze theories regarding the origin of life on Earth. Construct an argument from evidence supporting the idea that eukaryotes could not exist on the planet if not for prokaryotes. • Using information based on the geologic time scale and history of life on Earth, look for patterns in changes in organisms over time and explain how these patterns support the theory of evolution. • Use molecular data to construct cladograms depicting phylogenetic relationships between major groups of organisms. Procedure: Before starting agar art with living microbes/bacteria, keep all the following points in your mind: 1. Plan first. SAFETY ALWAYS! 2. Teach students how to streak a plate, how not to dig into the agar. 3. Give instructions on aseptic technique. 4. Make sure all students are compliant with safety rules, such as no dangling jewelry, no gum, tie back long hair, goggles, gloves, and aprons on. 5. Allow the plates to warm at room temperature (20 to 25°C), and the agar surface to dry before inoculating. 6. After agar is set, have students use a clean inoculation loop to plate the bacteria in the design they want, according to the colors they are looking for which come from the incubated bacteria. 7. Have the students put their agar dish with the lid ON under the incubator light. 8. Clean up lab benches using gloves, bleach and paper towels. AGAR PLATES SHOULD HAVE SET OVERNIGHT. Plan for days 2–3. 9. Observe agar plates! Take pictures, and explain to students how these are living art pieces. Bacteria will eventually take over the plate. 10. Properly dispose of bacterial samples used to plate, agar plates that are finished being used and inoculation loops in a ziplock bag with 1:1 ratio of bleach and water. This will ensure no cross contamination or illness leaves the lab. Enrichment: Modeling the art after a famous piece, utilizing bacteria to create certain colors needed. Push students who need enrichment to further question why certain bacteria metabolize certain colors Remediation: For students who need help, relate bacteria colors to the color in fruit. Help them understand that you cannot mix fruit to get a different color. Agar Art — An Intro to Microbiology (continued)

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