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+ ward ' s science Page 1 Agar Art — An Intro to Microbiology Submitted by: Lauren H. Recommended Grade Level(s): Appropriate for: Grade 9 Biology Time Requirements: Activity Time: 3 x 47 min class periods Teaching Topics & Concepts: • What cells are and how to distinguish between eukaryotes and prokaryotes. • How bacteria grow and reproduce. • Relate to Real Life: MRSA and Nosocomial infections National Science Standards Alignment Tennessee State Standards: BIO1.LS1: From Molecules to Organisms: Structures and Processes. BIO2.LS4: Biological Change: Unity and Diversity. Pre-Instruction: • Teacher: Pre-made TSA agar plates, gloves, goggles, apron, proper PPE, bacteria samples, heat lamp, sterile plastic inoculating loops, instruction manual, worksheets for students. • Technology: SmartBoard to show instructions on how to create a proper streak plate. • Student: writing utensil, hair tie (long hair), goggles, gloves, proper PPE equipment. Materials: For class: Agar plate This content is being brought to you by the Ward's World, Science By You program. This program brings lesson and classroom activity ideas directly from science teachers around the nation right to you. From best practices to activities, Science by You offers inspiration for every subject and every grade level. Interested in sharing your own content? Learn how today. PMS200 Black 70% Cool Gray 10 Black Astra Regular get for free from dafont.com • Microorganisms (microbes): 1. Bacillus subtilis (cream to brown) 2. Micrococcus luteus (yellow) 3. Chromobacterium violaceum (violet) 4. Escherichia coli (colorless) 5. Micrococcus roseus (pink) 6. Proteus mirabilis 7. Pseudomonas aeruginosa strains having variuous pigments i.e. pyocyanin, pyoverdin, prorubrin and pyomelanin 8. Serratia marcescens (pink or orange) 9. Staphylococcus aureus (yellow) 10. Vibrio fischeri (bioluminescent) 11. Yeast species — which are fungi — used include Saccharomyces cerevisiae (yellow-white) 12. Aspergillus flavus (yellow-green spores) 13. Aspergillus ochraceus (yellow) 14. Aureobasidium pullulans (black) 15. Candida species (whitish) 16. Candida albicans (whitish buff ) 17. Cladosporium herbarum (brown to black) 18. Cladosporium resinae 19. Epicoccum nigrum (yellow, orange, red, brown & black) 20. Fusarium species 21. Rhodotorula species and Scopulariopsis brevicaulis 22. Protist species used include Euglena gracilis (photosynthetic, green) and 23. Physarum polycephalum (yellow-green) • Inoculating loop • Bunsen burner • Incubator

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