Ward's World Working with Guides

Page 6 Culture Media 1. Bristol's Solution (as modified by H.C. Bold, Bull, Torrey Bot. Club 76: 101-108, 1949) Six stock solutions, 400 mL in volume, are employed. Each contains one for the following salts in the amounts listed: a) NaNO3 10.0 g d) K2HPO4 3.0 g b) CaCl2 1.0 g e) KH2PO4 7.0 g c) MgSO4 3.0 g f ) NaCl 1.0 g 10 mL of each stock solution are added to 940 mL of glass-distilled water. To this is added a drop of 1.0% FeCl3 solution. Two mL of minor elements solution (Trelease and Trelease, American Jour. Bot. 22: 520-542, 1935) may also be added. Solidify with 15 g agar per 1000 mL if desired. 2) Cyanophycean Agar (Culture Collection, Cambridge University) For each 1000 mL of medium required: KNO3 5.0 g K2HPO4 0.1 g MgSO4 • 7H2O 0.05 g Fe Ammonium Citrate 10 drops of 1% solution The above should be added to 1000 mL of Pyrex-distilled water. Solidify with 15g agar. 3) Desmid Agar (E.G. Pringsheim, Culture Collection, Göttingen) To 1000 mL of Pyrex-distilled water add 10 mL of each of the following solutions: 0.1% solution of MgSO4 • 7H2O 0.1% solution of K2HPO4 1.0% solution of KNO3 Solidify with 7.5 agar. It has been found that some desmid strains grow better with the addition of 50 mL/1000 mL of the supernatant from soil-water medium. 4) Erdschreiber Solution (Mary Parke, Plymouth Marine Station, England) 1000 mL filtered sea water 50 mL soil-water supernatant 0.2 g NaNO3 0.03 g Na2HPO4 • 12H2O 1st day: Filter seawater through No. 1 filter paper and then heat to 73°C. 2nd day: A) Again heat saltwater to 73°C. B) Autoclave salt solutions (made up in distilled water so that 1 mL of each solution gives required amount for 1000 mL of culture solution). 3rd day: Add cold salt solutions to cold soil supernatant, and then add soil supernatant to cold seawater. Dispense in sterile tubes and flasks as desired. 5) Euglena Medium (Culture Collection, Cambridge) To 1000 mL of Pyrex-distilled water add: Sodium acetate 1.0 g Beef extract 1.0 g Tryptone 2.0 g Yeast extract 2.0 g Calcium chloride 0.01g If desired, the above medium may be solidified by adding 15 g of agar. 6) Ochromonas Medium (W. Koch, Göttingen) To 960 mL of Pyrex-distilled water add: Glucose 1.0 g Tryptone 1.0 g Yeast extract 1.0 g Liver extract (infusion) 40 mL 7) Polytomella Medium (E.G. Pringsheim, personal communication) For each 1000 mL of medium required: Pyrex-distilled water 1000.0 mL Sodium acetate 2.0 g Yeast extract 1.0 g Tryptone 1.0 g 8) Porphyridium Agar (E.G. Pringsheim, personal communication) For each 500 mL of medium required: Pyrex-distilled water 200.0 mL Natural sea water 250.0 mL Soil-water supernatant 50.0 mL Yeast extract 0.5 g Tryptone 0.5 g Agar 7.5 g 9) Proteose Agar For each 1000 mL of medium required: Bristol's solution ([2], above) 1000.0 mL Proteose peptone 1.0 g Agar 15.0 g 10) Soil Extract Agar For each 1000 mL of medium required: Bristol's solution ([2], above) 960.0 mL Soil-water supernatant 40.0 mL Agar 15.0 g

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