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Page 8 D. Disposal of Nasonia and Sarcophaga Nasonia and Sarcophaga can be disposed of using two methods: 1. Create a "morgue" by partially filling a container with 10% isopropyl alcohol. Drop the Nasonia and Sarcophaga to be euthanized into the alcohol and cover the container with a lid. ~ OR ~ 2. Place Nasonia and Sarcophaga to be euthanized into a container and cover it with a lid. Place this container into a freezer and let it sit for at least two hours. 3. After euthanization, the container used for either method can be disposed of in a wastebasket. V. Haplodiploid Inheritance in Nasonia The unique reproductive strategy of Nasonia is known as haplodiploid inheritance and is also common to many other insects, such as bees. Nasonia exhibit a form of asexual reproduction, known as parthenogenesis, in which an organism is able to develop from an unfertilized egg. As a result, when the eggs are not fertilized, offspring will be haploid (n) and male. In turn, when the eggs are fertilized by a male, the chromosome number is restored to the diploid state (2n) and the offspring will be female (figure 7). Figure 7 Female (2n) Male (n) X Meiotic Egg Formation Meiotic Egg Formation Egg (n) Sperm (n) Fertilization Parthenogenesis Females (2n) Males (n)

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